Journal: Frontiers in Oncology

Article Title: MYD88 Is a Potential Prognostic Gene and Immune Signature of Tumor Microenvironment for Gliomas

doi: 10.3389/fonc.2021.654388

Figure Lengend Snippet: MYD88 was screened by the intersection of PPI network and univariate Cox analysis. (A) PPI network of the DEGs. (B) The top 30 proteins that had the maximum number of nodes in the PPI network. (C) Univariate Cox regression analysis for the survival of glioma patients. (D) The intersection analysis between the top 30 nodes in PPI network and the leading 33 genes ranked by the p-value of univariate Cox regression showed that MYD88 was the key gene.

Article Snippet: Peroxidase was blocked using the 3% H 2 O 2 for 25 min. Then we blocked the antigen using goat serum (Servicebio, G5001) for 30 min. We used MYD88 (1:20, CST, 4283S),CD68 (1:200, Servicebio, GB14043) and CD163 (1:600, Servicebio, GB13340) antibody overnight at 4°C to stain the slides, among which the two adjacent slides were stained with CD68 and CD163 separately.

Techniques:

Journal: Frontiers in Oncology

Article Title: MYD88 Is a Potential Prognostic Gene and Immune Signature of Tumor Microenvironment for Gliomas

doi: 10.3389/fonc.2021.654388

Figure Lengend Snippet: MYD88 was associated with the overall survival, age, WHO grade and enriched in immune pathways. (A) The MYD88 expression were significantly lower in normal tissues as compared to the glioma tissues. (B) MYD88 lower expression was associated with significantly longer overall survival. (C) The MYD88 expression was similar between male and female patients. (D) MYD88 expression was higher in patients with age > 52 years. (E) MYD88 expression was higher in patients with higher WHO grade. (F) The genes in MYD88 high-expression group were mainly enriched in immune related pathways. (G) For C7 collection defined by MSigDB, the immunologic gene sets, multiple immune functional gene sets were enriched in the high MYD88 expression group.

Article Snippet: Peroxidase was blocked using the 3% H 2 O 2 for 25 min. Then we blocked the antigen using goat serum (Servicebio, G5001) for 30 min. We used MYD88 (1:20, CST, 4283S),CD68 (1:200, Servicebio, GB14043) and CD163 (1:600, Servicebio, GB13340) antibody overnight at 4°C to stain the slides, among which the two adjacent slides were stained with CD68 and CD163 separately.

Techniques: Expressing, Functional Assay

Journal: Frontiers in Oncology

Article Title: MYD88 Is a Potential Prognostic Gene and Immune Signature of Tumor Microenvironment for Gliomas

doi: 10.3389/fonc.2021.654388

Figure Lengend Snippet: MYD88 was associated with the TIICs of TME in glioma patients. (A) The Macrophage M0, Macrophage M1 and Macrophage M2 were significantly up-regulated in MYD88 high-expression group. However, the Monocytes, Mast cells activated and Eosinophils were significantly up-regulated in MYD88 low-expression group. (B) The MYD88 expression was positively associated with Macrophage M0, Macrophage M1, and Macrophage M2. And MYD88 was negatively correlated with NK cell activated, Monocytes and Mast cells activated. (C) Intersection analysis showed that 14 TIICs were significantly associated with MYD88 expressions using both difference and correlation analysis.

Article Snippet: Peroxidase was blocked using the 3% H 2 O 2 for 25 min. Then we blocked the antigen using goat serum (Servicebio, G5001) for 30 min. We used MYD88 (1:20, CST, 4283S),CD68 (1:200, Servicebio, GB14043) and CD163 (1:600, Servicebio, GB13340) antibody overnight at 4°C to stain the slides, among which the two adjacent slides were stained with CD68 and CD163 separately.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: MYD88 Is a Potential Prognostic Gene and Immune Signature of Tumor Microenvironment for Gliomas

doi: 10.3389/fonc.2021.654388

Figure Lengend Snippet: Clinicopathological information of the glioma patients.

Article Snippet: Peroxidase was blocked using the 3% H 2 O 2 for 25 min. Then we blocked the antigen using goat serum (Servicebio, G5001) for 30 min. We used MYD88 (1:20, CST, 4283S),CD68 (1:200, Servicebio, GB14043) and CD163 (1:600, Servicebio, GB13340) antibody overnight at 4°C to stain the slides, among which the two adjacent slides were stained with CD68 and CD163 separately.

Techniques: Mutagenesis

Journal: Frontiers in Oncology

Article Title: MYD88 Is a Potential Prognostic Gene and Immune Signature of Tumor Microenvironment for Gliomas

doi: 10.3389/fonc.2021.654388

Figure Lengend Snippet: The MYD88 IHC staining of the 31 glioma patients. (A) MYD88 expression was significantly higher in high-grade gliomas. (B) In the IDH1 mutant cases, the MYD88 was significantly lower as compared to the cases with IDH1 wild cases. (C–F) IHC image of example glioma patients with WHO grade II/IDH1(+), WHO grade II/IDH1 (–), WHO grade III/IDH1 (–) and WHO grade IV/IDH1 (–) pathology results separately.

Article Snippet: Peroxidase was blocked using the 3% H 2 O 2 for 25 min. Then we blocked the antigen using goat serum (Servicebio, G5001) for 30 min. We used MYD88 (1:20, CST, 4283S),CD68 (1:200, Servicebio, GB14043) and CD163 (1:600, Servicebio, GB13340) antibody overnight at 4°C to stain the slides, among which the two adjacent slides were stained with CD68 and CD163 separately.

Techniques: Immunohistochemistry, Expressing, Mutagenesis